Abstract
Beneficial effects in bone cell growth and antibacterial action are currently attributed to Ga3+ ions. Thus, they can be used to upgrade mesoporous bioactive glasses (MBGs), investigated for tissue engineering, whenever they released therapeutic amounts of gallium ions to the surrounding medium. Three gallium-enriched MBGs with composition (in mol %) xSiO2–yCaO–zP2O5–5Ga2O3, being x = 70, y = 15, z = 10 for Ga_1; x = 80, y = 12, z = 3 for Ga_2; and x = 80, y = 15, z = 0 for Ga_3, were investigated and compared with the gallium-free 80SiO2–15CaO–5P2O5 MBG (B). 29Si and 31P MAS NMR analyses indicated that Ga3+ acts as network modifier in the glass regions with higher polymerization degree and as network former in the zones with high concentration of classical modifiers (Ca2+ ions). Ga_1 and Ga_2 exhibited a quick in vitro bioactive response because they were coated by an apatite-like layer after 1 and 3 days in simulated body fluid. Although we have not conducted biological tests in this paper (cells or bacteria), Ga_1 released high but non-cytotoxic amounts of Ga3+ ions in Todd Hewitt Broth culture medium that were 140 times higher than the IC90 of Pseudomonas aeruginosa bacteria, demonstrating its potential for tissue engineering applications.
Highlights
The inclusion of so-called therapeutic ions in silica-based mesoporous bioactive glasses (MBGs) is a subject of current interest because of the beneficial effects they can produce once implanted [1].The advantages given by these ions are connected to those of the traditional components of bioactive glasses: (i) stimulate the expression of genes of osteoblastic cells [2], (ii) stimulate angiogenesis [3],(iii) join certain antibiotics to produce antimicrobial behaviour [4], and (iv) show anti-inflammatory effects [5]
The main objective of this study is the correlation of the location of the gallium ions in the glass network with their bioactive behaviour, and the amount of Ga3+ ions released after soaking in common cell culture media, such as Dulbecco’s Modified Eagle Medium (DMEM) and Todd Hewitt Broth (THB)
The MBGs studied in this paper were obtained by evaporation-induced self-assembly (EISA)
Summary
The inclusion of so-called therapeutic ions in silica-based mesoporous bioactive glasses (MBGs) is a subject of current interest because of the beneficial effects they can produce once implanted [1]. (iii) join certain antibiotics to produce antimicrobial behaviour [4], and (iv) show anti-inflammatory effects [5]. In this context, Ga3+ ions are being investigated to be included in MBGs to obtain 3D scaffolds for bone regeneration—in particular for the defects treatment due to their propitious effects in bone cell growth and thanks to their antibacterial action. Gallium was already approved by the FDA for the treatment of hypercalcemia
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
