Abstract
BackgroundDespite the successful inhibition of human immunodeficiency virus type 1 (HIV-1) replication by combination antiretroviral therapy, cells latently infected with HIV-1 remaining in patients are a major obstacle for eradication of HIV-1 infection. The tumor suppressor factor p53 is activated by HIV-1 infection, and restricts HIV-1 replication. However, a therapeutic strategy based on p53 activity has not been considered for elimination of latently infected cells.MethodsApoptotic cells were analyzed using flow cytometry with anti-annexin A5-FITC Ab and PI staining upon treatment of anticancer drugs. The expression and activation of p53 and apoptotic molecules in latently HIV-1-infected T cells were compared using Western blot analysis. The role of p53 in the anticancer drug treatment-induced apoptosis of cells latently infected with HIV-1 was determined by knock-down experiment using siRNA against p53.ResultsUpon treatment with 5-fluorouracil (5-FU), apoptosis was increased in latently infected ACH2 cells encoding competent p53 compared with uninfected parent A3.01 cells, while the apoptosis of latently infected p53 null J1.1 cells was less than that of uninfected cells. Treatment with 5-FU increased the levels of cleaved caspase-3 and PARP in ACH2 cells compared with uninfected and latently infected p53 null J1.1 cells. The levels of expression and activation of p53 were higher in both latently infected ACH2 and NCHA2 cells than in uninfected cells. Furthermore, the activation levels of p53 in both cells were further increased upon 5-FU treatment. Consistent with p53 status, apoptosis was markedly increased in ACH2 and NCHA2 cells compared with uninfected and latently infected J1.1 cells upon treatment with other anticancer drugs such as doxorubicin and etoposide. Inhibition of p53 in cells with latent HIV-1 infection diminished apoptosis upon 5-FU treatment.ConclusionEvidence described here indicate that when treated with anticancer drugs, apoptosis of cells with latent HIV-1 infection was increased via the p53 activation pathway and may provide information for application of anticancer drugs to selectively eliminate HIV-1 reservoirs.
Highlights
Despite the successful inhibition of human immunodeficiency virus type 1 (HIV-1) replication by combination antiretroviral therapy, cells latently infected with HIV-1 remaining in patients are a major obstacle for eradication of HIV-1 infection
The anticancer drugs, 5-fluorouracil (5-FU), doxorubicin, and etoposide were purchased from Sigma-Aldrich (USA) and treated with the concentrations indicated in previous reports [16,17,18,19]
Distinct sensitivity of cells latently infected with HIV-1 to apoptosis upon 5-FU treatment numerous previous investigations have shown apoptosis of cells infected with HIV-1, apoptosis of latently infected cells is as yet little known
Summary
Despite the successful inhibition of human immunodeficiency virus type 1 (HIV-1) replication by combination antiretroviral therapy, cells latently infected with HIV-1 remaining in patients are a major obstacle for eradication of HIV-1 infection. One of the most innovative strategies aims to awaken the latent virus and thereby increase the killing of the latent infection reservoir by the immune system, known as “shock and kill”, and uses T-cell activating cytokines (IL-7), T-cell receptor signaling pathway activators (bryostatin and prostratin as a protein kinase C activator), histone deacetylase inhibitors (valproic acid, vorinostat (suberoylanilide hydroxamic acid)), DNA methylase inhibitors, and disulfiram [6]. These reactivating strategies have recently been shown to increase viral RNA in patients, reduction of reservoir size has not been consistently successful
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