Highly accurate and New approach for quantiϐication of Gramicidin in medication by RP-HPLC

Abstract

A significant Reverse Phase-High performance Liquid Chromatography technique was developed for a more accurate, unique and quick economical method was developed for the analysis of Gramicidin in medication dosage forms. The separation of this drug Gramicidin was done by using the X-Bridge phenyl column as a stationary phase, and a mixture of acetonitrile + buffer in 50:50 v/v ratio was used as a movable phase. The buffer used in this method was Octane sulphonic acid of pH-2.5 adjusted with OPA. The maximum absorbance of eluents was observed at 235 nm. A specific flow rate (1 ml/minute) was maintained throughout the runtime of 8 min. The selected drug is eluted at 2.49 minutes. The selected drug obeys Beer Lambert's law in the concentration range of 0.5-7.5 µg/ml of Gramicidin. The percentage of recovery was found to be within the acceptable limit. The selected approach was corroborated with ICH standard ground rules, and the results of parameters like method precision, accuracy, ruggedness, robustness, and degradation studies were found to be within the allowable limit. Thus, the present method was successfully applied for the simultaneous analysis of Gramicidin in routine industrial work.