High-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)

Abstract

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01). In BAP treatments, the maximum shoot-buds induction (9 shoot-buds per explant) and shoot regeneration percentage (96.66%) were observed on MS medium fortified with 2.2 and 4.4 µmol BAP in combination with 1 µmol of IAA. In TDZ treatments, the highest regeneration percentage was achieved in MS medium supplemented with TDZ (2.2 µmol) and IAA (1 µmol), and the maximum shoot-buds induction (19.83 shoot-buds per explant) was observed in medium containing 4.4 µmol of TDZ in combination with 1 µmol of IAA. The highest root production frequency (89.5%) was achieved in medium contained 9.84 µmol of IBA. Rooted plants were acclimatized successfully in greenhouse conditions with 100% survival. The protocol described here could be applicable for mass in vitro production of the valuable medicinal plant Hyssopus officinalis L. for its genetic resource conservation as well as pharmaceutical purpose.