Higher toxicity induced by co-exposure of polystyrene microplastics and chloramphenicol to Microcystis aeruginosa: Experimental study and molecular dynamics simulation

Abstract

Antibiotics and microplastics (MPs) inevitably coexist in natural waters, but their combined effect on aquatic organisms is still ambiguous. This study investigated the individual and combined toxicity of chloramphenicol (CAP) and micro-polystyrene (mPS) particles to Microcystis aeruginosa by physiological biomarkers, related gene expression, and molecular dynamics simulation. The results indicated that both individual and joint treatments threatened algal growth, while combined toxicity was higher than the former. Photosynthetic pigments and gene expression were inhibited by single CAP and mPS exposure, but CAP dominated and aggravated photosynthetic toxicity in combined exposure. Additionally, mPS damaged cell membranes and induced oxidative stress, which might further facilitate the entry of CAP into cells during co-exposure. The synergistic effect of CAP and mPS might be explained by the common photosynthetic toxicity target of CAP and mPS as well as oxidative stress. Furthermore, the molecular dynamics simulation revealed that CAP altered conformations of photosynthetic assembly protein YCF48 and SOD enzyme, and competed for functional sites of SOD, thus disturbing photosynthesis and antioxidant systems. These findings provide useful insights into the combined toxicity mechanism of antibiotics and MPs as well as highlight the importance of co-pollutant toxicity in the aquatic environment.