Higher specific activities of alkaline phosphatase and β-galactosidase for the cellular membrane integrity in young and old aged lithium treated rats

Abstract

Background: Differentiate the specific activity of Alkaline phosphatase and β-galactosidase in LiCl2 treated young and old rat's brain and liver to their control. Material and Methods: Totally 4 groups male wistar rats (each n=5) are taken to assess the level of Alkaline phosphatase and β-galactosidase for 10 and 20 days LiCl2 treated young and old rat's brain and liver as compared to control. Results: LiCl2 increases protein content essentail for old brain cell activation. Among all four group rats Alkaline phosphatase and β-galactosidase showed increased specific activity for 10 and 20 days LiCl2 treated young and old rat's brain and liver as compared to their control. Infact Alkaline phosphatase showed significantly higher activity for 10 and 20 days LiCl2 treated young brain (16.77 ± 0.309 and 27.74 ± 0.237) than their control 3.80 ± 0.230 and old rat's brain (14.28 ± 0.215 and 16.30 ± 0.24) than their control 8.65 ± 0.304 nm/min/mg of protein, similarly higher activity obtained for young liver (19.51 ±0.487 and 21.28 ± 0.317) than their control 3.67 ± 0.402 and finally for the old liver (15.02 ± 0.355 and 18.45 ± 0.439) as compared to their control 3.26 ± 0.304 nm/min/mg of protein respectively. Conclusion: These results confirm both membrane bound enzymes showed higher specific activity for LiCl2 treatment in brain and liver cells. So lithium has positive effect on young and old brain cells for β-galactosidase and has more positive effect on Alkaline phosphatase for rat liver and brain for membrane integrity of cells. Keywords: Total protein, Alkaline phosphatase, β-galactosidase, Specific activity, Lithium Chloride