Abstract
Thiosphaera pantotropha cells treated with mitomycin C produced bacteriophages and showed cell lysis. Upon occurrence of cell lysis, samples were mounted for electron microscopy by negative staining. During mounting, the cell contents were spread at the surface of the support film. Besides polysomes, strands interpreted as DNA could be seen, most of them complexed with particles interpreted as DNA-binding proteins. Single and twisted strands were revealed, and complex structures with diameters around 35 nm were common. They exhibited an ordered arrangement of the proteins. Our findings suggest that bacterial chromosomal DNA complexed with DNA-binding proteins may be organized in higher order, similar to the compactation of nucleosome strands in eukaryotic chromosomal DNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.