Abstract
High-efficiency plastome base editing in rice with TAL cytosine deaminase
Highlights
Genome editing has been widely applied in nuclei but not so in organelles such as the mitochondria and chloroplasts
We first designed an architecture of the transcription activator-like effectors (TALEs) DNA binding domain from a Xanthomonas oryzae TAL effector
The split halves (G1397-N and G1397-C) of DddAtox (Mok et al, 2020) each together with the uracil glycosylase inhibitor (UGI) were rice codon optimized and C terminally fused to the TALE scaffold (Supplemental Figure 2)
Summary
Genome editing has been widely applied in nuclei but not so in organelles such as the mitochondria and chloroplasts. The repeatless TALE scaffold contains an N terminus of 135 amino acids (aa), a 60-aa C terminus, and a cloning site between them for an insertion of designer TALE repeats of 34 aa, corresponding to the user-chosen target sequences (Supplemental Figure 1). A chloroplast transition peptide (cTP; Supplemental Figure 2) was fused N terminally to the TALE scaffold.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
