Abstract
Phase microscopy for high-dynamic-range quantitative phase-contrast imaging of a transparent phase object was demonstrated. Using a common path Fourier domain optical coherence tomography system, this technique is capable of displacement measurement with a sensitivity of 34 pm. The limitation of 2pi ambiguity restriction was overcome by the use of a phase retrieval approach performed in spectral domain. Two-dimensional quantitative phase imaging of human neonatal dermal keratinocyte cells was demonstrated to evaluate the performance of the system for cell imaging.
Highlights
High-sensitivity and high-speed quantitative phase measurement to retrieve nanometer or subnanometer scale variation is important for applications, such as subcellular dynamics studies and high resolution material inspection
The phase information is extracted from the complex depth-resolved profile, which is obtained by Fourier transformation of the spectral fringes
The measurement of optical path difference (OPD) with Fourier domain OCT (FDOCT) systems is restricted to less than half a wavelength owing to the 2π ambiguity
Summary
High-sensitivity and high-speed quantitative phase measurement to retrieve nanometer or subnanometer scale variation is important for applications, such as subcellular dynamics studies and high resolution material inspection. In phase measurement with FDOCT, the oscillation of the fringes in spectral domain due to optical path difference (OPD) between the reference and sample arms is detected.
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