Abstract

ObjectivesSalmonella bacteria can induce the unfolded protein response, a cellular stress response to misfolding proteins within the endoplasmic reticulum. Salmonella can exploit the host unfolded protein response leading to enhanced bacterial replication which was in part mediated by the induction and/or enhanced endo-reticular membrane synthesis. We therefore wanted to establish a quantitative confocal imaging assay to measure endo-reticular membrane expansion following Salmonella infections of host cells.Data descriptionHigh-content screening confocal fluorescence microscopic image set of Salmonella infected HeLa cells is presented. The images were collected with a PerkinElmer Opera LX high-content screening system in seven 96-well plates, 50 field-of-views and DAPI, endoplasmic reticulum tracker channels and Salmonella mCherry protein in each well. Totally 93,300 confocal fluorescence microscopic images were published in this dataset. An ImageJ high-content image analysis workflow was used to extract features. Cells were classified as infected and non-infected, the mean intensity of endoplasmic reticulum tracker under Salmonella bacteria was calculated. Statistical analysis was performed by an R script, quantifying infected and non-infected cells for wild-type and ΔsifA mutant cells. The dataset can be further used by researchers working with big data of endoplasmic reticulum fluorescence microscopic images, Salmonella bacterial infection images and human cancer cells.

Highlights

  • *Correspondence: j.kriston@ucl.ac.uk 5 Laboratory for Molecular Cell Biology, Medical Research Council, University College London, Gower Street, London WC1E 6BT, UK Full list of author information is available at the end of the article protein response (UPR), a cellular stress response to misfolding proteins within the endoplasmic reticulum (ER)

  • ER tracker staining was used for the quantification of endo-reticular membrane content in infected cells

  • Plate layouts Plate 1: A non-infected control plate was used, which contained HeLa cells were not infected with Salmonella enterica and were stained with varying endoplasmic reticulum (ER) tracker (ERT) concentrations

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Summary

Introduction

*Correspondence: j.kriston@ucl.ac.uk 5 Laboratory for Molecular Cell Biology, Medical Research Council, University College London, Gower Street, London WC1E 6BT, UK Full list of author information is available at the end of the article protein response (UPR), a cellular stress response to misfolding proteins within the endoplasmic reticulum (ER). We have established that following Salmonella infection of host epithelial cells, the unfolded protein response (UPR) is activated. We wanted to establish a confocal imaging method that could quantitatively determine endo-reticular membrane expansion, across a range of Salmonella infection conditions.

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