Abstract
The synthesis ofN-acetyllactosamine (D-Galpβ1-4D-GlcpNAc) with very low contamination of its isomerN-acetylallolactosamine (D-Galpβ1-6D-GlcpNAc) was obtained by use of regioselective transglycosylation activity of β-galactosidase fromBacillus circulansusing lactose as the donor of D-Galpand D-GlcpNAc as the acceptor. The reaction was conducted at 15°C and at pH 5.0. The incubation time was considerably reduced and the yield improved 100% with respect to the best results so far described in the literature.
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