High-Valent Silver-Porphyrin complex hybrid graphene oxide nanoplatform promotes MRSA-Infected wound healing

Abstract

The emergence of multidrug-resistant (MDR) bacteria represented by MRSA poses a great challenge to current anti-infection treatment. The traditional antibacterial agents containing Ag+ have been receiving intense attention due to their broad-spectrum activity. Compared with the Ag+, the high-valent silver ions including Ag2+ and Ag3+ have superior antibacterial ability. However, their extreme instability has severely limited their development as antimicrobials, and so far, no high-valent silver antimicrobials have been reported. Herein an unusual and water-soluble high-valent silver (II + III)-porphyrin complex (AgTMPyP) which can stably exist in an aqueous medium for at least 200 days, its corresponding composite AgTMPyP@GO was also successfully prepared. AgTMPyP@GO shows an excellent photothermal conversion rate (32.17 %) under excitation by an 808 nm near-infrared laser along with dual enzyme-like (peroxidase-like and oxidase-like) activities. When used for photothermal therapy, AgTMPyP@GO can effectively destroy biofilms and kill methicillin-resistant Staphylococcus aureus (MRSA) in vitro. The composite efficiently eradicates MRSA from wounds and rapidly promote wound healing in vivo. Moreover, hemolysis, blood routine, and blood biochemistry tests all demonstrate that AgTMPyP@GO has good biocompatibility. This work reveals that AgTMPyP@GO provides a synergistic antibacterial platform with effective antibacterial performance both in vivo and in vitro.