High-Throughput Validated Optical Sensor for the Dissolution Studies of Captopril Based on Instrumental-Free Detection through an Overhead Book Scanner

Abstract

A new high-throughput optical sensor for captopril (CAP) is proposed. The sensor is based on the inhibitory effect of the analyte on the specific reaction of desferal with Fe(III) in an acidic medium. The whole analytical scheme was developed in micro-plates format combined with instrumental-free detection through an overhead book scanner. Dissolution testing was selected as an interesting application due to the generation of large amounts of samples during drug development. The method allowed the quantification of CAP up to 500 μM, with a lower limit of quantification LLOQ of 25 μM. 96 samples/standards could be processed simultaneously, obtaining a complete dissolution profile within 1 h (n = 12). Following validation, the obtained results were in accordance with the HPLC method recommended by the US Pharmacopeia. Finally, the greenness of the procedure was assessed by well-established tools such as the Green Analytical Procedure and Blue Applicability Grade indexes (77.5% score).