Abstract
Abstract Surface-modified with tetraethoxysilane (TEOS) Superparamagnetic Nanoparticles (SNPs) were successfully synthesized to enhance their hydrophobicity and bio-affinity for an esterase. The obtained SNPs possessed large surface area, high conductivity and improved antimicrobial activity. An esterase from Bacillus pumilus was efficiently immobilized on silane functionalized SNPs and its successful binding was confirmed by both FTIR and FE-SEM. The low Michaelis Menten constant (Km) (∼12% lesser than free enzyme) of the SNPs-immobilized/bounded esterase (SNPs-Est) suggested its preeminent compatibility for the substrate (p-nitrophenyl acetate) than the free esterase. SNPs-Est retained half of its original activity even after 11.3 h of incubation and remained vigorous over 14 cycles of repetitive use at 45 °C. SNPs-Est was also checked out for successful synthesis of ethyl pyruvate (96% conversion of pyruvic acid into ethyl pyruvate), a flavor ester using High Performance Liquid Chromatography (HPLC) and 1H-Nuclear Magnetic Resonance (1H-NMR) studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
