Abstract
The transcriptomes of bread wheat Yunong 201 and its ethyl methanesulfonate derivative Yunong 3114 were obtained by next-sequencing technology. Single nucleotide variants (SNVs) in the wheat strains were explored and compared. A total of 5907 and 6287 non-synonymous SNVs were acquired for Yunong 201 and 3114, respectively. A total of 4021 genes with SNVs were obtained. The genes that underwent non-synonymous SNVs were significantly involved in ATP binding, protein phosphorylation, and cellular protein metabolic process. The heat map analysis also indicated that most of these mutant genes were significantly differentially expressed at different developmental stages. The SNVs in these genes possibly contribute to the longer kernel length of Yunong 3114. Our data provide useful information on wheat transcriptome for future studies on wheat functional genomics. This study could also help in illustrating the gene functions of the non-synonymous SNVs of Yunong 201 and 3114.
Highlights
Wheat (Triticum aestivum L.) is one of the three most important cereals, with more than 600 million tons harvested annually (Shewry, 2009)
This study provided important information to further understand the transcriptome of hexaploid wheat and determine the wheat-specific genes related to grain size
The first Single nucleotide variants (SNVs) type was that the mutation sites were same between Yunong 201 and 3114, and the mutation was non-synonymous as compared with reference sequence, but the nucleotides after mutation were different between Yunong 201 and 3114, we considered this mutation significant
Summary
Wheat (Triticum aestivum L.) is one of the three most important cereals (i.e., maize, rice, and wheat), with more than 600 million tons harvested annually (Shewry, 2009). Some quantitative trait loci (QTLs) controlling grain shape and size in bread wheat have been mapped through marker analysis in different wheat populations (Breseghello and Sorrells, 2006; Wu et al, 2015). GRAIN SIZE 3 (GS3) is a major QTL for rice grain length and weight (Fan et al, 2006), TaGS-D1 was the syntenic gene in wheat (Zhang et al, 2014). These studies revealed that grain size of bread wheat is regulated via a complex molecular genetic mechanism. Next-generation sequencing (NGS) technology (Goff et al, 2002) provides a novel method to identify, map, and quantify transcriptomes (Kyndt et al, 2012); this method can be used for rapid characterization
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
