High-throughput sequencing-based analysis of the composition and diversity of the endophytic bacterial community in the roots of Dipsacus asperoides.

Abstract

This study employed Illumina high-throughput sequencing technology to investigate diversity and community structure of endophytic bacteria in wild D. asperoides growing in three distinct regions. The study analyzed the impact of region on endophytic bacteria, uncovered the core bacterial community, and furnished valuable insights for the screening of endophytic bacteria. This study identified 6,540 amplicon sequence variants (ASVs) coexisting with D. asperoides roots. These ASVs belong to 35 phyla, 84 classes, 204 orders, 365 families, and 708 genera. At the phylum level, the dominant phyla were Proteobacteria and Actinobacteria, while at the genus level, Acidothermus, Acidibacter, Bradyrhizobium, Frankia, and Pseudomonas emerged as the dominant genera. Furthermore, noticeable differences in endophytic bacterial communities were observed between the Yunnan and Guizhou regions. These findings can serve as a reference for the authentication of medicinal materials from various origins and the selection of active strains.