Abstract
Pea (Pisum sativum L.) is a model plant that has been used in classical genetics and organ development studies. However, its large and complex genome has hindered research investigations in pea. Here, we generated transcriptomes from different tissues or organs of three pea accessions using next-generation sequencing to assess single nucleotide polymorphisms (SNPs), and further investigated petal differentially expressed genes to elucidate the mechanisms regulating floral zygomorphy. Eighteen samples were sequenced, which yielded a total of 617 million clean reads, and de novo assembly resulted in 87,137 unigenes. A total of 9044 high-quality SNPs were obtained among the three accessions, and a consensus map was constructed. We further discovered several dorsoventral asymmetrically expressed genes that were confirmed by qRT-PCR among different petals, including previously reported three CYC-like proliferating cell factor (TCP) genes. One MADS-box gene was highly expressed in dorsal petals, and several MYB factors were predominantly expressed among dorsal, lateral, and/or ventral petals, together with a ventrally expressed TCP gene. In sum, our comprehensive database complements the existing resources for comparative genetic mapping and facilitates future investigations in legume zygomorphic flower development.
Highlights
Pea (Pisum sativum L.) has long been regarded as a classical model legume in genetics studies since Mendel’s time
Virus-induced gene silencing (VIGS) involving the bean pod mottle virus (BPMV) and pea early browning virus (PEBV) induces gene silencing in accession JI992, thereby allowing the functional analysis of pea and other legumes [1,4,5]
Our studies have demonstrated that conducting virtual map-based cloning in pea could be an efficient approach in comparative genomic mapping, which is largely dependent on identifying markers in the corresponding regions [2,15]
Summary
Pea (Pisum sativum L.) has long been regarded as a classical model legume in genetics studies since Mendel’s time. Unlike A. majus, whose IN asymmetry is mainly controlled by DICH [26], our previous study favored an idea that a new locus in pea, SYMMETRICAL PETALS1 (SYP1), is responsible for the establishment of IN asymmetry in both lateral wings and ventral keels [1]. These data suggest that the molecular mechanisms regulating legume floral zygomorphy might be different from A. majus, and a transcriptome-wide understanding of genes involved in the morphogenesis of different petals could be an alternative way to address this issue. Our comprehensive database complements the existing resources in comparative genetic mapping and better facilitates research studies on legume zygomorphic flower development
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