Abstract
Fortification of effective microbes (EM) such as Pediococcus acidilactici and photosynthetic bacteria in fertilizer and animal feed exhibiting effective outcomes in enhancing crops and livestock production as well as upholds sustainable agriculture concept. However, the productivity of the EM biomass stocks is unsurpassed to the demand due to non-optimized media. In this study, two sets of nutrient pair comprising of beef extract-manganese and yeast extract-glucose for P. acidilactici and photosynthetic bacteria respectively were studied. The optimum media formulated was based on the highest growth rate in 96-microwell plate system were subsequently tested in 1 L cultures volume for EM biomass production. Results showed that growth rate and dry weight of P. acidilactici in media supplemented with 64 g/L beef extract (0.145 h-1, 0.243 g) and 0.28 g/L manganese (0.1134 h-1, 0.32 g) were higher compared to control medium with an increment of 17.39 % and 54.59 % biomass productivity as compared to MRS medium respectively. Meanwhile, the growth rate and dry weight for Photosynthetic bacteria in media supplemented with 6.63 g/L glucose (0.2538 h-1, 0.167 g) and 0.44 g/L yeast extract (0.2733 h-1, 0.163 g) recorded 11.3 % and 8.73 % biomass productivity increment in comparison to the HCH medium.
Highlights
Effective microorganism (EM) is a mixture of liquid inoculant bacterial culture comprising of photosynthesizing bacteria, lactic acid bacteria, yeast, actinomycetes and fermenting fungi that can upsurge the microbial diversity in the soil ecosystem
We provide a new method of improving cultivation medium using high throughput screening based - Automated Media Optimization System
A new improved cultivation medium for Pediococcus acidilactici and Photosynthetic bacteria was successfully formulated based on Screen 1 data and tested in 1 L flask culture in order to determine the growth and biomass of the effective microbe cultures
Summary
Effective microorganism (EM) is a mixture of liquid inoculant bacterial culture comprising of photosynthesizing bacteria, lactic acid bacteria, yeast, actinomycetes and fermenting fungi that can upsurge the microbial diversity in the soil ecosystem. There are many benefits of using EM including enhancing the process of decomposing organic microorganism and other compound other than suppressing soil-borne pathogens. The photosynthetic bacteria play a leading role in EM biotech industry as they are commonly used as an additive for biofertilizer to improve the quality of the crops. This is due to the ability of photosynthetic bacteria in nitrogen fixation, sulphide oxidation and carbon sequestration in various natural environments [3]
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