High-throughput, modular and logic analysis platform based on binary photonic crystals array and entropy-driven amplifier for multiplex biomarkers detection

Abstract

The development of high-throughput and modular assay of multiplex biomarkers has great significance for disease diagnosis but it still faces some difficulty. Herein, a high-throughput, modular and logic analysis platform combining binary photonic crystals (PCs) array and entropy-driven DNA amplifier (EDA) was developed to achieve simple, high-sensitive, and nonenzymic detection of multiplex biomarkers. In this platform, three modules including logic-recognition, EDA and binary PCs array were integrated, and each module had independent and universal design but connected functions. The binary PCs array was fabricated conveniently through stacking self-assembly of two kinds of colloidal nanospheres, and could specifically enhance two kinds of fluorescence based on the match between emission peak and photonic band gap. Functionally, the input of two mRNA biomarkers initiated the logical operation of logic-recognition module, and the released DNA strand triggered corresponding EDA module for amplified fluorescence signal output (FAM or Cy5). The whole DNA circuit was performed in one step. Then, binary PCs array module further enhanced both two fluorescence signals, and got direct fluorescence signal readout by microplate reader (scanning rate of 96 samples/ 7 min). It is the first time that the binary PCs array-based high-throughput platform has achieved logical and sensitive detection of multi-biomarkers by employing logic gate and EDA. Therefore, the proposed platform has merits of logical analysis, high sensitivity, programmable design, high throughput and multiplex detection, showing potential application in bioanalysis and clinical diagnostics.