Abstract
Interleukin-6 (IL-6), an immune protein in the hematopoietins family, has been demonstrated as a prognostic indicator for survival in patients with gastric carcinoma. In this contribution, we introduced a new and high-throughput electrochemical immunosensor for the detection of IL-6 in human. The immunoassay protocol was fabricated by the immobilization of anti-IL-6 antibodies on the nanogold and partial ferrocenyltethered dendrimer (Fc-D)-functionalized interface. A sandwich-type immunoassay format was employed for the IL-6 determination using horseradish peroxidase (HRP)-labeled anti-IL-6 as secondary antibody. The properties and factors influencing the performance of the proposed immunosensor were evaluated in detail. Under optimal conditions, the current response of the formed sandwich-type immunocomplex relative to H2O2 system was proportional to IL-6 concentration ranging from 2.5 pg/mL to 120 pg/mL with a detection limit of 1.0 pg/mL (at 3δ). The precision, reproducibility and stability of the immunosensor were acceptable. Subsequently, the immunosensors were used to assay the IL-6 in human serum specimens. Analytical results were in agreement with those obtained by the standard chemiluminescence ELISA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.