Abstract
We recently reported a sugar-induced bacterial release of 13-Docosenamide and its ability to quench fluorescein. This simple handle to monitor bacterial growth is readily applicable to develop a quicker antibiotic sensitivity testing method along with a low-cost field-use optical instrumentation. Conditions were standardized to perform this new procedure in the most preferred and CLSI-recommended microdilution format in 12-well strips. A simple and portable optoelectronic prototype was used to capture the image and read the fluorescence signal of the culture medium of the 12-well strips. This new Fluorescence Quenching Method along with the device enabled the choice of the right antibiotic within 8 h of sample collection from the patient. It was compliant to the Clinical Laboratory Standard Institute’s quality control guidelines. Clinical assessment of the method using 440 urine samples from Urinary Tract Infection patients against 21 routinely used antibiotics showed a 94.3% match with the results of the Standard Disk Diffusion method. This new method saves the precious time taken for and the cost of antibiotic susceptibility testing for quicker and effective treatment with better compliance.
Highlights
Among the many factors attributed to the rise of antibiotic resistance, prescription of antibiotics without susceptibility testing (AST) is the primary and the most alarming one[1]
Fluorescence Quenching Method (FQM) results matched Clinical and Laboratory Standards Institute (CLSI) quality control range for four reference American Type Culture Collection (ATCC) bacterial strains
Recommended reference ATCC bacterial strains against a panel of 10 different antibiotics, prepared according to the MIC ranges defined by CLSI (Table 1)
Summary
Among the many factors attributed to the rise of antibiotic resistance, prescription of antibiotics without susceptibility testing (AST) is the primary and the most alarming one[1]. We had recently published that Escherichia coli and 26 other bacteria associated with human diseases when grown in glucose-containing medium, released long-chain fatty amide(s) of the type 13-docosenamide, which quenched fluorescein, a widely-used inexpensive fluorophore. This quenching of the fluorescence of fluorescein was directly proportional to the growth of bacterial culture[6]. An absence in the fluorescence of the growth medium indicates bacterial growth while the fluorescence of the uncultured medium remains intact We adapted this bacterial growth monitoring technique that based on the optical shift, for developing a simpler and quicker
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