Abstract
Postmenopausal osteoporosis (PMOP) is a common clinical illness in postmenopausal women, but there is no effective drug at present. Metabolomics approach was used to explore the potential biomarkers of PMOP and evaluate the efficacy and therapeutic targets of total lignans in the stem of Acanthophanax senticosus (ASSL) on the ovariectomized osteoporosis model rats. UPLC/MS and pattern recognition methods were used for serum metabolites discovery to illustrate the pathological mechanism of PMOP model rats, and then revealing the intervention effect of ASSL. The pattern recognition result showed that serum metabolic profiles of the sham operation group and the model group were clustered clearly, and 16 potential biomarkers were finally identified (7 in positive ion mode and 9 in negative ion mode), and they are involved in 15 related metabolic pathways. After oral administration of ASSL, 10 biomarkers were found to be significantly up-regulated and mainly regulated metabolic pathways include unsaturated fatty acid biosynthesis, linoleic acid metabolism, and arachidonic acid metabolism, primary bile acid synthesis, tyrosine metabolism, etc. Our study demonstrated that the ASSL could affect the endogenous metabolites related metabolic mechanism, provides a pharmacological basis of the ASSL for PMOP treatment.
Highlights
Osteoporosis (OP) is a systemic bone disease characterized by bone mass reduction, bone strength reduction, and bone microstructure degeneration with the aging and aging of the human body, and it is the main cause of high fracture incidence in recent years, which can lead to bone fragility and increased fracture risk (Wang et al, 2018; Watts, 2018)
Alkaline phosphatase kit and tartrate-resistant acid phosphatase kit were purchased from the Institute of Bioengineering (Nanjing, China); Osteocalcin kit was purchased from Northern Biotechnology Research Institute (Beijing, China); sodium chloride injection was acquired from Sanlian Pharmaceutical Co., Ltd. (Harbin, China); iodophor disinfectant was purchased from Xinruida Disinfectant Co., Ltd. (De zhou, China); chloral hydrate and sodium carboxymethyl cellulose were obtained from the Institute of Photosynthetic Fine Chemicals (Tianjin, China); penicillin sodium powder injection from Harbin Pharmaceutical Group Pharmaceutical Factory; acetonitrile, methanol, acetone were obtained from Merck; Neil Estrone was purchased from Vikchi Biotech Co., Ltd. (Sichuan, China); other reagents and chemicals used were of analytical grade
Compared with the NYL, ASSLH, and ASSLM groups, the increase in bone density was similar, indicating that all three groups can improve bone mineral density reduction caused by ovariectomy, improve bone load capacity, and increase bone biomechanical properties
Summary
Osteoporosis (OP) is a systemic bone disease characterized by bone mass reduction, bone strength reduction, and bone microstructure degeneration with the aging and aging of the human body, and it is the main cause of high fracture incidence in recent years, which can lead to bone fragility and increased fracture risk (Wang et al, 2018; Watts, 2018). It has irreversible characteristics, and its early intervention methods have become a problem and focus of the whole society.
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