High-Throughput, Green, Low-Cost, and Efficient Recovery of Heparin from a Biological Mixture Using Bio-Originated Magnetic Nanofibers

Abstract

Heparin is an anticoagulant commercially derived from porcine intestinal mucosa that is challenging to selectively isolate for further purification. In this study, we propose the fabrication of amine-functionalized magnetic nanobiofibers (NBF@Fe-NH2), 1–2 μm long and 50–200 nm wide with mostly tube-shaped structures, originating from grape seeds as green and efficient bioadsorbents for the efficient recovery of heparin. To rapidly screen for heparin adsorption, we used a novel high-throughput technique based on UV–vis spectroscopy and 96-well microplates. The heparin uptake performance of the fabricated adsorbents vs Amberlite FPA98 Cl, a conventional absorbent utilized in industry for heparin recovery, was examined in a mixture containing heparin isolated from porcine intestinal mucosa. The NBF@Fe-NH2 showed a higher heparin uptake perf compared to the Amberlite FPA98 Cl. Furthermore, we also performed a sheep plasma clotting assay and found that the heparin sample separated by NBF@Fe-NH2 featured a high...