Abstract
We report a novel inexpensive and up-scalable fabrication technique for viable hepatocyte clusteroids and demonstrate that they grow faster than individual cells in tissue engineering applications.
Highlights
Culturing of cells as three-dimensional (3D) clusters can enhance in vitro tests for basic biological research as well as for therapeutics development
Isolated tumour cells have been cultured into individual globules in these DEX drops and their response to drug therapy has been further studied[12,20,21,22] since higher chemotherapeutic drug resistance has been observed when globules manufactured using this technique are compared to 2D cultured globules.[19]
We demonstrate that the clusteroids fabricated by our w/w Pickering emulsion templating enable the hepatic cells to gain higher growth rate, which results in clearly enhanced albumin and urea production
Summary
Adverse liver manifestations are greatly valued in the pharmaceutical sector[7] as well as other industries. Das et al reported a rapid, expandable technique for the production of tissue globules from human embryonic kidney cells (HEK293) by trapping them in w/w Pickering emulsion droplets.[23,24]. Their technique is based on the formation of w/w Pickering emulsions, in which cells are efficiently distributed in emulsion droplets In their method, the interfacial tension of the aqueous DEX phase in the droplets, where the cells partition preferentially, is used to wrap the cells in separate compartments, and the droplets are shrank by changing the osmotic balance of the two aqueous phases (ATP), rapidly driving the cells from larger and loosely packed drops to mostly spherical cell clusters. We demonstrate that the clusteroids fabricated by our w/w Pickering emulsion templating enable the hepatic cells to gain higher growth rate, which results in clearly enhanced albumin and urea production
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