Abstract
High separation temperatures in liquid chromatography and liquid chromatography-mass spectrometry with a porous graphitic carbon column were investigated. Separation temperature was varied up to 200 degrees C, and the effect on retention, analysis time, and sensitivity was measured. Analysis times were reduced more than six-fold, whilst baseline resolution was maintained. The impact of the separation temperature on signal-to-noise ratio with atmospheric pressure chemical ionisation or electrospray mass spectrometric detection was also investigated. The potential of using superheated water for the analysis of some very polar compounds is illustrated. Monitoring of column stability detected no loss of performance, due to the highly stable nature of the 100% carbon stationary phase.
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