Abstract
The enzyme-membrane integrated system has become an attractive method for the depolymerization of lignocellulosic biomass. This research was planned to evaluate the effect of pH, temperature, enzyme loading, and reaction time in order to maximize the sugar production. Hydrolysate extracted from pineapple leaves was used as a substrate. The feed substrate was hydrolyzed by β-xylosidase under different working conditions using the one factor at a time (OFAT) method. The best working conditions, obtained via enzymatic hydrolysis were used in the enzyme-membrane integrated system. OFAT results revealed that the best working conditions were pH 7.5, 50 °C, 1% (w/v) and 30 mins. Sugar yield at simultaneous reaction and filtration was higher (293.94%) than the reaction alone (32.23%).
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