High-Speed Optical Sorting of Soft Wheat for Reduction of Deoxynivalenol.

Abstract

Fusarium head blight (FHB) is a fungal disease that affects small cereal grains, such as wheat and barley, and is becoming more prevalent throughout much of the world's temperate climates. The disease poses a health risk to humans and livestock because of the associated production of the mycotoxin deoxynivalenol (DON or vomitoxin) by the causal organism, Fusarium graminearum. A study was undertaken to examine the efficiency of high-speed, optical sorting of intact wheat (Triticum aestivum) kernels for reduction of DON concentration. Soft red winter (n = 32) and soft white (n = 3) wheat samples, known to have elevated levels of FHB, were obtained from commercial mills throughout the eastern United States. An additional seven samples of wheat from the discard piles of in-mill cleaners were also studied. Fusarium-damaged wheat, cleaned of nonkernels and foreign material ( ~4.5 kg/sample, DON range = 0.6 to 20 mg/kg), was fed into a commercial high-speed bichromatic sorter operating at a throughput of 0.33 kg/(channel-min) and a kernel rejection rate of 10%. A wavelength filter pair combination of 675 and 1,480 nm was selected for sorting, based on prior research. Visual measurements of the proportion of Fusarium-damaged kernels were collected on incoming and sorted seed (separate analyses of accepted and rejected portions), as were measurements of DON concentration. Results indicated that the fraction of DON contaminant level in the sorted wheat to that in the unsorted wheat ranged from 18 to 112%, with an average of 51%. Nine of the 35 regular samples and all seven of the discard pile samples underwent a second sort, with five from this second set undergoing a third sort. Multiple sorting was effective in producing wheat whose DON concentration was between 16 and 69% of its original, unsorted value.