Abstract
A significant limitation of fiber bundle endomicroscopy systems is that the field of view tends to be small, usually only several hundred micrometers in diameter. Image mosaicking techniques can increase the effective image size, but require careful manipulation of the probe to ensure sufficient overlap between adjacent frames. For confocal endomicroscopes, which typically have frame rates on the order of 10 fps, this is particularly challenging. In this paper we demonstrate that line-scanning confocal endomicroscopy can, by use of a high speed linear CCD camera, achieve a frame rate of 120 fps while maintaining sufficient resolution and signal-to-noise ratio to allow imaging of topically stained gastrointestinal tissues. This leads to improved performance of a cross-correlation based mosaicking algorithm when compared with lower frame-rate systems.
Highlights
Confocal endomicroscopy is an endoscopic technique for obtaining fluorescence microscopy images in vivo [1, 2]
We report a high speed, line-scanning, fiber bundle endomicroscope which uses a 1D line-scan camera
While the system can be used with any fiber bundle, for this work we used a Coloflex UHD probe taken from a Cellvizio endomicroscope
Summary
Confocal endomicroscopy is an endoscopic technique for obtaining fluorescence microscopy images in vivo [1, 2]. Higher frames rates (on the order of 10 fps) can be more readily achieved by generating the scanning pattern in bulk optics and relaying it to the tissue by a flexible fiber imaging bundle and (optionally) a distal micro-objective [5, 6]. In this case the number of useful ‘pixels’ of information is limited by the number of fiber cores (typically 30,000), necessitating a trade-off between lateral image size and resolution
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