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Abstract
We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.
Highlights
The delivery of target molecules by liquid flows has been often utilized in various biosensing systems
The reactions were conducted for 90 min, and the fluorescence images of both substrates were obtained in phosphate-buffered saline (PBS) solution by a fluorescence microscope
Since fluorescence intensities are correlated with the amount of bound FITC-labeled streptavidin molecules on the biotinylated substrates, the results indicate that the biotinylated substrate could effectively capture streptavidin molecules, as we expected
Summary
The delivery of target molecules by liquid flows has been often utilized in various biosensing systems. A liquid sample containing the analyte of interest was loaded onto one end of the kit, and the sample flowed through various zones of receptor-coated strips As another example, in a lab-on-chip system, the analyte solutions flow through micro channels, which conduct a series of reaction processes, including the pretreatment of samples and the binding reaction between the targets and their receptors [2]. In a lab-on-chip system, the analyte solutions flow through micro channels, which conduct a series of reaction processes, including the pretreatment of samples and the binding reaction between the targets and their receptors [2] In these examples, the lateral flow of the analyte solutions is mostly used for the delivery of the analyte solution to the desired location of the sensor. The non-specific binding of molecules could lead to a decrease in the sensitivity of biosensors by increasing the background noise of sensor signals [4]
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