High-Speed Imaging of Broadband Multiplex Coherent Anti-Stokes Raman Scattering Microscopy Using a Supercontinuum Source

Abstract

We combined broadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy with CARS microscopy using a femtosecond laser and a photonic crystal fiber. Broadband multiplex CARS microspectroscopy, which simultaneously measures broadband range (including significant Raman vibrational modes) from 500 to 3500 cm-1 at a high spectral resolution was subsequently performed. Also, via multiplex CARS microscopy using a pair of galvanometer mirrors we successfully demonstrated the high-speed imaging of polymer beads and a lily pollen grain for the study of cell biology dynamics. The imaging speed was 37 s per image, which is 30–40 times faster than that previously reported in broadband CARS microscopy.