Abstract
A new rapid micellar electrokinetic chromatographic (MEKC) method has been developed for the determination of two kinds of coumarin (aesculin and aesculetin) inCortex fraxini. After a series of optimization experiments, 100 mM boric acid (pH 9.5), 50 mM sodium dodecyl sulfate (SDS), 30 kV applied voltage and 35°C temperature were selected. The contents of aesculin and aesculetin inCortex fraxini were successfully determined within 6 min by this method with quercetin as internal standard. The relative standard deviations (RSD) of the method are 1,22% for aesculin and 1.02% for aesculetin on the basis of peak areas. The detection limits (3S/N) are 25 μg mL−1 for aesculin and 20 μg mL−1 for aesculetin. Calibration curves are linear in the concentration ranges of 136–1020 μg mL−1 for aesculin (r=0.9969) and 126–945 μg mL−1 for aesculetin (r=0.9993), and the recoveries are 98.5% for aesculin and 98.6% for aesculetin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
