Abstract
The purpose of this study was the production of maltobionic acid, in the form of sodium maltobionate, by Z. mobilis cells immobilized in polyurethane. The in situ immobilized system (0.125-0.35mm) was composed of 7g polyol, 3.5g isocyanate, 0.02g silicone, and 7g Z. mobilis cell, at the concentration of 210g/L. The bioconversion of maltose to sodium maltobionate was performed with different cell concentrations (7.0-9.0 gimobilized/Lreaction_medium), temperature (30.54-47.46°C), pH (5.55-7.25), and substrate concentration (0.7-1.3mol/L). The stability of the immobilized system was evaluated for 24h bioconversion cycles and storage of 6months. The maximum concentration of sodium maltobionate was 648.61mmol/L in 34.34h process (8.5 gdry_cell/Lreaction_medium) at 39°C and pH 6.30. The immobilized system showed stability for 19 successive operational cycles of 24h bioconversion and 6months of storage, at 4°C or 22°C.
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