Abstract

A total of 552 serum samples of sheep, goats and cattle from different localities at Assiut governorate, Egypt were screened for qualitative analysis of the BTV antibodies using a commercial competitive ELISA (cELISA) kit. The results showed an overall percentage of BTV positive sheep, goats and cattle serum samples were 41.86%, 24% and 85.42% respectively. A highest percentage of seropositivity was found inArab El- Awamer farm (100%), followed by Abnoub( 63. 64%), Sedfa(11. 43%), veterinary teaching hospital (11.11%), Aboteeg (3.57%) and Bani zeed el akrad (0% ) respectively. From examined sera all over the four years from 2012 to 2015 indicates prevalence of BTV antibodies by ( 62. 69%) during the non hot months and (10%) during the hot months, Female ruminants were more susceptible to the infection (61.29%) than male( 21.67%). The association between sex and prevalence of BT disease among ruminants observed in table (4). The results showed that the prevalence of BT was higher in female (61.29%) than male (21.67%). In conclusion, this study confirms the presence of blue tongue infection in Assiut Governorate, and cELISA were found to be sensitive and effective for screening of BTV group specific antibodies. The disease should be notifying its presence in Egypt since it was free from this disease and more studies should be attempets to estimate the serotype of blue tongue virus

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