Abstract
Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature. The study of cardiovascular disease typically involves imaging of the large coronary vessels and quantification of cardiac blood perfusion. These methods, however, are not well suited for imaging of the cardiac microvasculature. We used the optical histology method, which combines chemical optical clearing and optical imaging, to create high-resolution, wide-field maps of the cardiac microvasculature in ventral slices of mouse heart. We have demonstrated the ability of the optical histology method to enable wide-field visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease.
Highlights
The coronary vasculature is the network of cardiac blood vessels that delivers blood and other nutrients to the heart to maintain its normal function
Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature
We have demonstrated the ability of the optical histology method to enable widefield visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease
Summary
The coronary vasculature is the network of cardiac blood vessels that delivers blood and other nutrients to the heart to maintain its normal function. A loss of blood flow to the heart due to dysfunction of the coronary vasculature leads to damage and death of the cardiac muscle cells, resulting in angina and myocardial infarction. Methods to quantify blood perfusion in the heart include computed tomography [2], position emission tomography (PET) [3], ultrasound [4], and magnetic resonance imaging (MRI) [5,6]. Methods to visualize the cardiac vasculature include coronary angiography [7], PET [8,9,10], intravascular ultrasound [11,12,13], and MRI [14]
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