Abstract
Single molecule detection using biological nanopores in lipid bilayers is crucially limited by noise and bandwidth of the recording. We have tested a newly developed 16-channel microelectrode cavity array (MECA, Ref. 1) for single molecule detection using alpha-Hemolysin (alphaHL) nanopores. The device is based on subpicoliter cavities in a high-quality dielectric polymer adding less than 0.5 pF to the input capacitance of the amplifier (Axopach200B), thereby optimizing noise and bandwidth.View Large Image | View Hi-Res Image | Download PowerPoint SlideAn example trace is shown in Fig.1A with an open state rms noise of 0.65pA at 0-5 kHz. Note that for the blocked state, there is no significant difference between an all points histogram of a 5 kHz filtered trace (black) and that of a event amplitudes defined by averaging (grey). Fig.1B shows 35 single PEG induced blockages superimposed and aligned in time. They are detected as square pulses down to durations<100µs and longer events seem to correlate with deeper blocks. The recording performance of MECAs with high integration densities and superior mechanical stability is expected to greatly facilitate single molecule nanopore analysis in the future.(1)Baaken et al.(2008),Lab Chip 8(6):938-44.
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