Abstract

We have developed a tomographic diffractive microscope in reflection, using a high numerical aperture objective and equipped with a fluorescence confocal scanner. We describe the set-up and first images of a microscopic USAF target, obtained in holographic, diffractive tomographic, and confocal mode, and which reveal the higher resolution capabilities of this instrument. We also compare images obtained in transmission and in reflection, emphasizing the better optical sectioning capabilities of reflection diffractive tomographic microscopy.

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