Abstract
Neuromelanin is a dark coloured intracellular pigment that appears in a specific population of neurons (dopaminergic and noradrenergic) predominantly in the substantia nigra and in the locus coeruleus. In recent years, there is increasing interest in the role of neuromelanin because of a hypothesised link between this pigment and the cell death of neuromelanin-containing neurons in Parkinson’s disease (PD). Therefore, the role of neuromelanin in the pathology of PD is an actual focus in neuroscience. We have investigated the elemental contents and distributions of sulphur, calcium, iron, nickel and copper of neuromelanin in dopaminergic neurons for a Parkinson case and a control case ( in situ, 6 μm brain sections). There was no difference in the iron concentration between the two cases. However, the calcium concentration was 3-fold higher in the Parkinson case, whereas the copper and nickel concentrations decreased. An ultrastructural investigation of the concentrations of calcium and iron within the neuromelanin suggests that these two elements are not necessarily co-localized.
Highlights
IBIL ICAM 1 Ig IHC IL IRE Iron-Responsive Element-Binding Protein 1 (IRP1) Kat
Die für die immunhistochemischen und histochemischen Färbungen verwendeten Chemikalien und Reagenzien sind in den nachfolgenden Tabellen mit ihrem jeweiligen Hersteller und in ihrer Zusammensetzung aufgelistet
Der ungebundene Antikörper konnte wiederum durch dreifaches Waschen in PBS-T, wobei die Spülzeit bei jedem Gang sieben Minuten betrug, entfernt werden
Summary
Eisen stellt im menschlichen Körper ein essentielles Übergangsmetall dar. Weiterhin ist es an diversen Redox-/Nicht-Redox-Reaktionen, am Sauerstofftransport sowie dem Elektronentransfer (Gonzalez et al 2016; Wong et al 2012; Rassow 2012; Löffler 2008) beteiligt und stellt einen Cofaktor in der Synthese zahlreicher Proteine und Nucleinsäuren dar (Löffler 2008; Rassow 2012). Im Gehirn spielt Eisen beispielsweise als Cofaktor der Tyrosin-Hydroxylase im Rahmen der Dopamin- und Noradrenalinsynthese eine entscheidende Rolle (Klinke et al 2005) und ist für die Myelinisierung der Axonen bestimmend
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