Abstract
To find a promising molecular method for meat traceability, three methods of single nucleotide polymorphism (SNP) detection: RFLP-PCR analysis, high-resolution melting (HRM) analysis, and TaqMan probe analysis, have been compared in terms of accuracy, ease of use, throughput capability, and cost. We genotyped ten pork DNA samples across three SNPs. The results showed that the HRM genotyping method was the most accurate and easiest to use with the lowest cost, while TaqMan probe analysis provided similar results, but its cost was much higher.
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