Abstract
Bryonia laciniosa L. (Family: Cucurbitaceae) is commonly known as ‘Shivlingi’ and is a highly valued medicinal plant in Ayurveda and Homeopathy systems of medicine particularly as a remedy for infertility. In the present study, sophisticated, highly reliable, and sensitive high resolution-mass spectrometry (HR-MS) was carried out for the determination of chemical constituents present in B. laciniosa . Phytochemical screening of methanolic extract showed the presence of saponins, sterols, triterpenoids, flavonoids, alkaloids, phenolic compounds, and tannins. TLC of sterol-rich petroleum ether extract (SRPE) produced six major spots on precoated silica gel GF 254 , using toluene: methanol (96:04 v/v) as mobile phase. The spots showed green, purple, light purple to violet colour spots in the Rf range of 0.2-0.91, after derivatization with the anisaldehyde-sulphuric acid reagent. Further, based on TLC, HR-MS analysis of SRPE was performed for the identification of phytoconstituents, at positive ESI (electron spray ionisation) mode. It indicated the presence of a total of 30 compounds including short fragments of peptides. The major compounds predicted in HR-MS: Q(quadrupole)-TOF(time-of-flight)-MS, as per METLIN database, were swietenine, ergosterol acetate, 4, 4, dimethyl-14α-formyl-5α-cholesta-8,24-dien-3β-ol, L-olivosyl-oleandolide, mitoxantrone, isogedunin, 3S-aminodeconoic acid, and nisoldipine.
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