Abstract
Milk serves as a mode of protection to neonate through transferring the host defense proteins from mother to offspring. It also guards the mammary gland against various types of infections. Along with the presence of six vital proteins, bovine milk (whey) contains a massive class of minor proteins, not all of which have been comprehensively reported. In this study, we performed an LC-MS/MS-based ultra-deep identification of the milk whey proteome of Indian zebu (Sahiwal) cattle. Three independent search engines that are Comet, Tandem, and Mascot-based analysis resulted in the discovery of over 6,210 non-redundant proteins commonly identified. Genome-wise mapping revealed that chromosome 1 showed a minimum expression of 14 proteins, whereas chromosome 19 expressed 250 maximum proteins in milk whey. These results demonstrate that milk proteome in Sahiwal cattle is quite complicated, and minor milk fractions play a significant role in host defense.
Highlights
Milk synthesis is an absolute feature that defines the mammalian class
The present study aims to determine the comprehensive profile of bovine milk whey proteome in Indian zebu (Sahiwal) cattle
An outstanding feature of analyzing this dataset is the conversion of open file format and its interchange data processing between modules under the umbrella of Trans Proteomics Pipeline (TPP)
Summary
Milk synthesis is an absolute feature that defines the mammalian class. It has received considerable interest because of its nutritional and functional properties to newborn and young offspring [1]. It majorly acts as a vital source for the transfer of defense molecules against pathogens from mother to child. Milk acts as a medium for the transfer of host defense proteins, yet its repertoire of minor proteins has been only partly characterized [2]. Bovine milk is exceedingly being utilized for dairy products, including yogurt, curd, cheese, butter, and, to some extent, as bioactive peptides [3]
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