Abstract
Key messageQTLstm9controlling rapid-onset water stress tolerance inS. habrochaiteswas high-resolution mapped to a chromosome 9 region that contains genes associated with abiotic stress tolerances.Wild tomato (Solanum habrochaites) exhibits tolerance to abiotic stresses, including drought and chilling. Root chilling (6 °C) induces rapid-onset water stress by impeding water movement from roots to shoots. S. habrochaites responds to such changes by closing stomata and maintaining shoot turgor, while cultivated tomato (S. lycopersicum) fails to close stomata and wilts. This response (shoot turgor maintenance under root chilling) is controlled by a major QTL (designated stm9) on chromosome 9, which was previously fine-mapped to a 2.7-cM region. Recombinant sub-near-isogenic lines for chromosome 9 were marker-selected, phenotyped for shoot turgor maintenance under root chilling in two sets of replicated experiments (Fall and Spring), and the data were used to high-resolution map QTL stm9 to a 0.32-cM region. QTL mapping revealed a single QTL that was coincident for both the Spring and Fall datasets, suggesting that the gene or genes contributing to shoot turgor maintenance under root chilling reside within the marker interval H9–T1673. In the S. lycopersicum reference genome sequence, this chromosome 9 region is gene-rich and contains representatives of gene families that have been associated with abiotic stress tolerance.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-015-2540-y) contains supplementary material, which is available to authorized users.
Highlights
Abiotic stresses, especially those which affect the water relations of the plant such as low temperatures, may decrease plant growth and yield
The chromosomal location of stm9 in our study agrees with Goodstal et al (2005) who fine-mapped stm9 to marker interval T1670–T1673 (~2.7 cM)
We refined the location of stm9 to marker interval H358–T1673, a genetic distance of 0.32 cM
Summary
Especially those which affect the water relations of the plant such as low temperatures, may decrease plant growth and yield. The susceptibility to water stress induced by root chilling in species of tropical and sub-tropical origin is a concern for agricultural production in Mediterranean climates such as California, where exposure to cold soils in the spring can affect seedling establishment because soil temperatures under an open canopy may be colder than air temperatures (Nobel 1999). Cultivated tomato (Solanum lycopersicum) is a classic example of a chilling-sensitive crop (Geisenberg and Stewart 1986). It was domesticated from the wild cherry tomato, which is native to mesic, tropical environments (Rick 1983). A related wild tomato species, S. habrochaites, grows in the Peruvian Andes at altitudes up to 3300 m and
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