Abstract
Abstract Development of high resolution liquid chromatography can be traced to the use of columns with particle size of less than 30 μm (1). As anticipated from theoretical considerations, this has produced a significant improvement in resolution over previously used larger particle size columns; therefore, based on the subjective judgement of the chromatographer, the technique is designated either high performance or high resolution liquid chromatography. Discussion is provided below as to what is currently considered high resolution and how one might reasonably define it.
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