Abstract
AbstractThe immunogold-silver technique was modified so that 1–2 μm thick glycolmethacrylate sections could routinely be used for high resolution immunocytochemistry. A rabbit antihuman hemoglobin antibody was used to demonstrate the distribution of exogenous hemoglobin in mouse tissues. Inclusion of 0.1% glutaraldehyde in the primary formalin fixation improved tissue appearance. Glutaraldehyde treatment after colloidal gold antibody application increased the staining intensity, and gold chloride intensification of the silverenhanced colloidal gold further improved immunosensitivity. Thus, a more accurate analysis of hemoglobin distribution in tissue was obtained. The enhancements were also beneficial for localizing myelin basic protein in brain and for determining the proliferative status of tissue by localization of bromodeoxyuridine incorporation into nuclei.Manual capillary action staining efficiency was demonstrated during all steps in the immunogold-silver staining protocol, greatly simplifying the ...
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
