Abstract

Comparative chromosome banding analysis and/or fluorescence in situ hybridization (FISH) studies are established approaches to compare human and ape chromosomes. FISH banding is a relatively new and not routinely applied method very well suited to provide to a better understanding of the evolutionary history of primate and human phylogeny. Here multicolor banding (MCB)-applying probes derived from Homo sapiens were used to analyze the chromosomes of Thai crab-eating macaque (Macaca fascicularis). The results agree with those of previous studies in other macaques, e.g. Macaca sylvanus or Macaca nemestrina. This result highlights that morphological differences within the Cercopithecoidea must be found rather in subchromosomal changes or even in epigenetics than in gross structural alterations. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS220504012E">10.2298/ABS220504012E</a><u></b></font>

Highlights

  • Since recently next-generation sequencing (NGS) was introduced to answer the question what the genetic differences between species are, molecular cytogenetic studies to contribute to a better understand of the evolutionary history of primate and human phylogeny seem to be outdated

  • The most frequently applied fluorescence in situ hybridization (FISH)-banding approach is the so-called multicolor banding (MCB), which has the unique feature of be

  • Macaca fascicularis (MFA) was previously studied by banding cytogenetics (Fernandez-Donoso et al, 1970, Kanagawa et al, 1971; Brown et al, 1986); to the best of our knowledge, FISH was only applied for single loci (Archidiacono et al, 1998; Kasai et al, 2000; Kostova et al, 2002; Ruiz-Herrera et al, 2004; Liu et al, 2007) and not for the whole genome

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Summary

INTRODUCTION

Since recently next-generation sequencing (NGS) was introduced to answer the question what the genetic differences between species are, molecular cytogenetic studies to contribute to a better understand of the evolutionary history of primate and human phylogeny seem to be outdated. MFA was previously studied by banding cytogenetics (Fernandez-Donoso et al, 1970, Kanagawa et al, 1971; Brown et al, 1986); to the best of our knowledge, FISH was only applied for single loci (Archidiacono et al, 1998; Kasai et al, 2000; Kostova et al, 2002; Ruiz-Herrera et al, 2004; Liu et al, 2007) and not for the whole genome. We provide the first genome-wide MCB-based FISH-banding study in MFA

MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION

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