High-resolution imaging of the interplay of replicative proteins on DNA using correlative tweezers fluorescence microscopy

Abstract

DNA is synthesized by an orchestrated multi-protein machinery in a highly coordinated and dynamic fashion. Current single-molecule strategies provide us great details in kinetic motion of replicative proteins and dynamic DNA-protein interaction. However, many of these studies lack the possibility for direct observing multi-molecules interacting with DNA. Here we present a Correlative Tweezers Fluorescence Microscopy (CTFM) strategy to observe, with high-temporal-resolution, multiple replicative protein interacting on single DNA molecules.