Abstract
Double-stranded poly(dG)–poly(dC) and triple-stranded poly(dG)–poly(dG)–poly(dC)DNA were deposited on the modified surface of highly oriented pyrolitic graphite(HOPG) and visualized using atomic force microscopy with high-resolution (radius of∼1 nm) tips. The high resolution attained by this technique enabled us to detectsingle-stranded regions in double-stranded poly(dG)–poly(dC) and double-strandedand single-stranded regions in poly(dG)–poly(dG)–poly(dC) triplexes, as wellas to resolve the helical pitch of the triplex molecules. We could also followthe reaction of G-strand extension in poly(dG)–poly(dC) by the Klenowexo− fragment of DNA polymerase I. This approach to molecular visualization could serve as auseful tool for the investigation of irregular structures in canonical DNA and otherbiopolymers, as well as studies of the molecular mechanisms of DNA replication andtranscription.
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