High-resolution analysis of anti-migratory inhibitors in high-grade glioma treatment

Abstract

Glioblastoma Multiforme (GBM) is an extremely aggressive form of glioma tumour. The characteristically invasive properties of GBM contribute to the poor prognosis in sufferers. This study focuses on the therapeutic potential of four anti-migratory drugs: 6-bromo-indirubin-3’-oxime (BIO), CCG-1423, Latrunculin A (LAT A) and Lithium Chloride (LiCl), in vitro. The migration distances of GBM cells and cell circularity for each treatment and an untreated control were measured and statistically analysed. This was achieved using treated GBM spheroids of the U251 cell line and an untreated control. Spheroid sections were analysed in QuPath. This data was analysed in SPSS. Each of the treatments showed significant statistical differences compared to the control spheroid for the migration distance measurements. LAT A was shown to have the greatest effect in decreasing the cell migration. Migrated cell circularity were significantly more rounded than the non-migrated cells for each treatment. The cell migration distance study suggests further study, to analyse the effects of these drugs in vivo. The ‘cell circularity index’ study should be amended to more accurately represent the changes in cell elongation due to morphological changes occurring as cells migrate from the tumour.