High-resolution 3D light-field imaging.

Qiang Geng,Zhiqiang Fu,Shih-Chi Chen

doi:10.1117/1.jbo.25.10.106502

Qiang Geng, Zhiqiang Fu + Show 1 more

Open Access

https://doi.org/10.1117/1.jbo.25.10.106502

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Abstract

.Significance: High-speed 3D imaging methods have been playing crucial roles in many biological discoveries.Aim: We present a hybrid light-field imaging system and image processing algorithm that can visualize high-speed biological events.Approach: The hybrid light-field imaging system uses the selective plane optical illumination, which simultaneously records a high-resolution 2D image and a low-resolution 4D light-field image. The high-resolution 4D light-field image is obtained by applying the hybrid algorithm derived from the deconvolution and phase retrieval methods.Results: High-resolution 3D imaging at a speed of 100-s volumes per second over an imaging field of in the , , and axis, respectively, is achieved with a 2.5 times enhancement in lateral resolution over the entire imaging field compared with standard light-field systems. In comparison to the deconvolution algorithm, the hybrid algorithm addresses the artifact issue at the focal plane and reduces the computation time by a factor of 4.Conclusions: The new hybrid light-field imaging method realizes high-resolution and ultrafast 3D imaging with a compact setup and simple algorithm, which may help discover important applications in biophotonics to visualize high-speed biological events.

Highlights

A large m value can directly improve the lateral resolution of the reconstructed images, especially at the focal plane
11 2D images from z 1⁄4 −40 to 40 μm with 8 μm intervals are generated via deconvolution, where the image at the focal plane is replaced by the 2D image from scientific complementary metal-oxide-semiconductor (sCMOS) 1
One may observe the lateral resolution has been improved over the entire scanning range and the effect becomes more prominent with increasing distances away from the focal plane, i.e., the resolution of the hybrid Light-field microscopy (LFM) remains relatively constant over the range in comparison with standard LFM

Summary

Introduction

Visualization of 3D high-speed biological events, e.g., signaling of neural circuits, has been the driving force of many emerging 3D imaging techniques, such as random-access microscopy[1,2] and light-sheet microscopy (LSM).[3,4] The former approach selectively scans the laser beam in the regions of interest to monitor biological events at speeds up to 10-s kHz,[1,2] and the latter approach exploits a light sheet to parallelly illuminate and record 2D images at frame rates up to 100-s Hz;[3,4] clear 3D images can be obtained through the application of customdesigned phase masks[3] or synchronization with an axial scanner,[4] achieving a volume image rate of ∼30 Hz. Light-field microscopy (LFM)[5] is a high-speed 3D imaging technique that captures a volume image via a single exposure from a camera, where the imaging speed is limited by cameras.

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