High rates of nitrogen fixation of Ulex species in the understory of maritime pine stands and the potential effect of phosphorus fertilization

Abstract

Nitrogen (N2) fixation by Ulex species was studied in a range of mature maritime pine (Pinus pinaster Ait.) stands as well as in a phosphorus (P) fertilization trial in a young (6-year-old) open pine stand. The biomass was estimated by allometric relationships, and the percentage of N derived from atmosphere was calculated according to the natural 15N abundance method. Ulex stand biomass was lower in the mature pine stand than in the young open pine stand. In the latter pine stand, Ulex mean annual biomass increment ranged from 1 to 5 Mg·ha–1·year–1, increasing with P fertilization dose, as did the Ulex stand biomass, ranging from 5.4 to 31.1 Mg·ha–1 after six growing seasons in the most highly fertilized treatment. For the sites where the natural 15N abundance method was applicable, the calculated percentage of N in the Ulex europaeus L. tissues derived from atmosphere was very high (mean = 82%; range = 59%–100%). At the other sites, the N2 fixation was probably also very important but could not be calculated with confidence. Here, the mean fixation rate of the former sites was used to calculate the N2 fixation flux. The estimated annual N2 fixation flux ranged from 0.5 to 5.1 kg N·ha–1·year–1 in the mature pine stands. In the young open pine stand, the Ulex understory fixation ranged from 8.1 to 57.4 kg N·ha–1·year–1, increasing with P fertilization dose. Considering the low levels of N fluxes in theses ecosystems, these figures are very high.