Abstract
The phospholipid analogue with two short fatty chains, 1-dodecyl-2-[1- 14C] octanamido- sn-2-deoxy-gly cero-3-phosphocholine ([ 14C] phospholipid analogue), with a non-hydrolyzable bond at position 2 of the glycerol, is an inhibitor of phospholipase A 2. It was obtained after chemical synthesis and 0.5 μmol was solubilized in Na +taurocholate with an equimolar amount of 1-octadecanoyl 2-[ 3H]eicosatetraenoyl- sn- glycero-3-phosphocholine which is the current substrate of phospholipases A 2. Both molecules were introduced into the duodenum of rats in order to follow their captations by intestinal mucosa cells for 30, 60 or 90 min. The [ 14C] phospholipid analogue was poorly split by phospholipases A 2(pancreatic juice and intracellular enzymes). It disappeared from the intestinal contents (87% of the dose gone in 90 min) as rapidly as the tritiated lecithin (81%) but this was later split by the phospholipases at a higher rate.
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